Determination of Ursolic Acid in Compound Flavone Capsule by HPLC
Abstract:ObjectiveTo establish the method for determining the content of ursolic acid in Compound Flavone Capsules .MethodsThe C18 column with methanol- water- phosphorous acid (90∶10∶0.03) as the mobile phase was used. The flow rate was 0.5 ml/min and the detective wavelength was 220 nm . ResultsThe linear range of calibration curve was 0.982~9.82 μg/ml (r = 0.999 8). The average recovery was 98.32% and the RSD was 0.56% (n= 6).ConclusionThe method is simple and accurate with a good reproducibility. It can be used for the quality control of Compound Flavone Capsules.