Abstract:ObjectiveTo develop a quality standard for super micropowder of notoginseng.Methods Qualitative analysis of micropowder of notoginseng was carried out by TLC. The particle diameter was detected with laser scattering particle size distribution anaslyzer. An RP - HPLC method was used for the content determination of the Notoginsensode R1, ginsenoside Rg1, ginsenoside Rb1. ResultsThe chromatographic spots of micropowder of notoginseng were identified without the interference of negative control. Micropowder of particle average diameter was below 15μm. Notoginsensode R1 had a good linearity in the range of 0.535~3.210 μg (r = 1.000) ,and the average recovery was 103.08% with RSD of 2.80%. Ginsenoside Rg1 had a good linearity in the range of 2.225~13.350 μg (r=1.000) ,and the average recovery was 100.33 % with RSD of 2.29 %. Ginsenoside Rb1 had a good linearity in the range of 2.205~13.230 μg ( r= 1.000) ,and the average recovery was 101.00% with RSD of 1.43%. ConclusionThis method is simple ,accurate and repeatable. It can be used to determine ginsenoside Rg1,ginsenoside Rb1and notoginsensode R1 in micropowder of Notoginseng.
Key words:Micropowder of Notoginseng; Particle diameter; HPLC; TLC; Quality standard