Abstract:ObjectiveTo compare the extraction yield of volatile oil and menthol from Mentha haplocalyx Briq. by different extraction methods. MethodsThe volatile oil was extracted by steam distillation, cold-soaked, ultrasound, supercritical carbon dioxide extraction, and the content of menthol was detected by GC. ResultsThe extraction yield of volatile oil and menthol for supercritical carbon dioxide extraction were 2.43% and 1.77%, for ultrasound extraction were 1.34% and 1.09%, for cold-soaked extraction were 1.27% and 1.02%, for steam distillation extraction were 1.15% and 0.90%. ConclusionSFE-CO2 is the optimal method to extract volatile oil from Mentha haplocalyx Briq..