Abstract:ObjectiveTo observe the changes of lymphocyte subsets in the peripheral blood of mice with immunological liver injury and intervention of Garlic Polysaccharide C. MethodsThe animal immunological liver damage model was reproduced by injection via the tail vein of BCG (Bacilli Calmette Guein)and lipopolysaccharide(LPS) into mice.The peripheral-blood lymphocyte subsets' percentages and the CD4+/CD8+ ratio was measured by the flow cytometry.ResultsCompared with normal control group, the percentages of the T lymphocyte and the CD8+ T lymphocyte in the peripheral-blood in immunological liver damage mice siginificantly increased(P< 0.01),however, the percentages of the B lymphocyte, CD4+T lymphocyte and the ratio of CD4+/CD8+ decreased(P<0.05 or P< 0.01). Garlic polysaccharide C at dose of 800 mg/kg (ig,qd×12) decreased the percentages of T,B,CD4+ T and CD8+ T lymphocyte in the peripheral-blood in mice treated with BCG+LPS, however,the CD4+/CD8+ ratio increased to the normal level. The percentages of the NK1.1+T lymphocyte showed no obvious difference between control, BCG+LPS liver injury model and Garlic Polysaccharide C groups.ConclusionGarlic Polysaccharide C can inhibit the immunologic activity, and establish a new immunologic balance, thereby produce protective effects on immunological liver injury.
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