Abstract:ObjectiveTo determine the content of Hypoxanthine in Pheretima Compounding Granules.MethodsAn RP-HPLC was established.The chromatographic column was Inertsil ODS-EP.The mobile phase was H2O-CH3OH-C4H8O(100∶0.5∶0.5).The flow rate was 1.0 ml·min-1 ,and the detection wavelength was 254 nm. ResultsHgpoxanthine showed good linear relationship in the range of 0.040 6~0.406 0μg. The average recovery for Hypoxanthine was 97.6% and RSD was 0.63%(n=9).ConclusionThis method is simple, accurate and can be used for content determination of Pheretima Compounding Graunles.