Abstract:ObjectiveTo determine irisflorentin in cultivated Belamcanda chinensis(L.)DC by HPLC,and to inspect the quality of its ingredients.MethodsUsing HPLC with TSK ge1 ODS Column80(250 mm×4.6 mm),the mobile phase was composed of a mixture of methanol-phosphoric aicd aqueous soluton (pH=3)∶53%∶47%,the flow rate was 1 ml/min,the UV detection wavelength was set at 265 nm,and the temperature was at 35℃.ResultsIrisflorentin showed linear relationship with peak areas in the range of 0.0118~0.118 μg(r=0.999 9).The average recovery was 99.84%,RSD was 2.509%.ConclusionThe method is simple,rapid and accurate.The quantity of irisflorentin in Cultivated Belamcanda Chinensis(L.)DC exceeds 0.10% and meets the Chinese Pharmacopoeia.
Abstract:ObjectiveTo determine irisflorentin in cultivated Belamcanda chinensis(L.)DC by HPLC,and to inspect the quality of its ingredients.MethodsUsing HPLC with TSK ge1 ODS Column80(250 mm×4.6 mm),the mobile phase was composed of a mixture of methanol-phosphoric aicd aqueous soluton (pH=3)∶53%∶47%,the flow rate was 1 ml/min,the UV detection wavelength was set at 265 nm,and the temperature was at 35℃.ResultsIrisflorentin showed linear relationship with peak areas in the range of 0.0118~0.118 μg(r=0.999 9).The average recovery was 99.84%,RSD was 2.509%.ConclusionThe method is simple,rapid and accurate.The quantity of irisflorentin in Cultivated Belamcanda Chinensis(L.)DC exceeds 0.10% and meets the Chinese Pharmacopoeia.